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ARTOS 3D

Get consistent, ultrathin serial sections for array tomography in less time with the ARTOS 3D ultramicrotome

The ARTOS 3D (ARray TOmography Solution) automatically creates and collects hundreds of serial-section ribbons all ready for array tomography with your scanning electron microscope (SEM).

Save time and effort in biological sample preparation and SEM setup procedure so you can quickly get the images you need to answer critical research questions.

Faster Array Tomography

How does the ARTOS 3D ultramicrotome allow you to save time when making high-quality sections for array tomography?

Array tomography (AT) is a 3D image reconstruction technique for high-resolution, quantitative analysis of biological structures. For optimal results, ultrathin, ordered sections are a pre-requisite.

Until now, AT has involved several time-consuming and cumbersome manual steps. The ARTOS 3D solution speeds up the process by automating specimen sectioning and minimizing the time required to align the sections for SEM imaging.

Read on to find out how ARTOS 3D helps you speed up the workflow while also achieving high-quality results.

 

Fast Sectioning & Easy Alignment for Imaging ARTOS 3D vs. Conventional Ultramicrotome

Less Work for You with the ARTOS 3D Workflow

To obtain excellent results with array tomography in a timely manner, it is crucial to create ultrathin, consistent, and correctly ordered ribbons efficiently. To achieve this advantage, the ARTOS 3D ultramicrotome automates making high-quality, ultrathin (>20 nm) sections. The automation eliminates several of the manual work steps required by more conventional ultramicrotomes, such as the EM UC7 also from Leica Microsystems. This automation leads to large time-savings for serial sectioning and ribbon alignment before SEM imaging.

The ARTOS 3D system minimized or eliminated the manual work steps required with conventional ultramicrotomes. There is:

  • No manually aligning nor positioning of the ribbon sections with an eyelash
  • No manual handling of the ribbons multiple times for repeat sectioning which increases the risk of wrinkling
  • No need to perform over and over the same tedious manual process to get wrinkle-free ribbons on the section carrier

 

Save Time by Making High-Quality Sections with a Seamless Workflow

Fast section preparation with much less effort

With the ARTOS 3D ultramicrotome, you have a seamless workflow through the whole preparation process by eliminating manual work steps:

  • Fast setup with programs pre-defined by the user for different section carriers
  • Automated creation and collection of hundreds of serial sections with minimal user intervention
  • No more time-consuming and fiddly manual collection involving sorting and positioning of ribbons to attain proper alignment for SEM imaging

Reproducible, Artifact-Free Sections

Consistent high-quality sections

The ARTOS 3D ultramicrotome can deliver reproducible, high-quality sections rapidly by:

  • Avoiding the artifact-causing conditions of manual sectioning and manipulation due to integrated direct section-ribbon collection
  • Collecting ultrathin section ribbons without wrinkling by easily adjusting the water flow with the front valve
  • Minimizing variation in section thickness by eliminating air turbulence and vibration thanks to a especially-designed draft shield and active damping plate
  • Sectioning ribbons precisely with the custom-designed 4 mm diamond knife, adaptable to different section carriers

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