Leica EM GP2
The updated version of the EM GP automatic plunge freezer is based on our motto ‘with the users for the user’ in combining performance and flexibility in a practically relevant way.
The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane after removing excess fluid by automatic blotting.
Prior to freezing the sample is maintained in a temperature and humidity-controlled environmental chamber, adjustable between +4°C and +60°C, and room humidity to 99 %.
Reproducible Results Within Minutes
Micrographs A and B show images of microtubules acquired at 20000x (A) and 39000x (B) nominal magnification in the files of the perforated carbon film. The ice is free of exogenous contamination, the protofilament and the individual tubulin subunits are clearly visible along the length of the microtubules.
Courtesy of Dr. Guenter Resch, Nexperion E.U. - Solutions for Electron Microscopy; Dr. Thomas Heuser and Marlene Brandstetter, Vienna, Biocenter Core Facilities GmbH
User Safety
Safe and reproducible preparation for single particle analysis and cryo-tomography.
- Special rapid condensing liquefier head for easy and safe full of secondary cryogen
- Fixed secondary cryogen position, only environmental chamber movement
Reproducibility
Freezing grids reproducibly and safely using the EM GP2 automatic plunge freezer
- Sensor blotting gives an equal distributed surface on the grid for perfect imaging results
- Temperature stability of secondary cryogen
- Temperature and humidity-controlled protected environment
Connectivity
To be connected at any place is today something which is taken for granted
As a logical consequence, Leica Microsystems connected systems for EM Sample Preparation to ensure it protected your samples during the complete workflow process
Sample Safety
- Dry Nitrogen Gas Flow environment
- Sample Storage and Transfer in the liquid face using transfer container with pre-cooled grid box
- Customized adapter for optimized and safe sample transfer from the automatic plunge freezer EM GP2 to different cryo TEMs
Flexibility
Depending on your needs the EM GP2 automatic plunge freezer can be configured individually by using different blotting types and parameters:
- Sensor controlled blotting
- Automated or manual single, double, and multiple blotting
- Environmental parameters – Temperature and Humidity
- Blotting Parameter – Pre-Blot, Blot, Post-Blot time, Blot, and Transfer Positions
Operating Conditions |
Min/Max |
Ideal |
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Room Temperature |
+15 to +30 °C |
+21 to +24 °C |
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Room Rel. Humidity |
Max. 55 % |
Around 40 % |
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Storage Temperature |
+5 to +35°C |
+21 to +24 °C |
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Storage Humidity |
Max. 55 % |
Around 40 % |
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Room Ventilation |
Required at all times during operation. |
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Environmental Chamber |
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Temperature Control |
+4 to +60 °C |
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Humidity Control |
Room Humidity to 99% |
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Chamber Features |
Light (LED), Anti-Fog Heating |
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Dewar |
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LN2 Dewar Volume |
1 l |
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Dewar Temperature |
-196 °C |
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Dewar bake-out Temperature |
Max. +110°C |
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LN2 Consumption |
~ 1 l/h1 |
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| 1TF 50%, Dewar covered with lid, secondary cryogen temperature -180°C | ||
Preparation Chamber |
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Allowed Secondary Cryogen |
Ethane / Propane |
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Secondary Cryogen Container Temperature |
0 to -196 °C |
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Secondary Cryogen Container Volume |
2.5 ml |
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